Donia H. Embarez

Conference 2024 Live Talk

Talk Title

Two novel streptomyces strains for paracetamol-traces bioremediation with chemoinformatics, safety examination of biodegradation products

Authors and Affiliations

Donia H. Embarez1, Ahmed S. Abdel Razek2(the late), Emad B. Basalious3, Magdi Mahmoud1, Nadia M. Hamdy 4
1 Biochemistry Department, Faculty of Science, Ain Shams University, Abassia, 11566, Cairo, Egypt, doniahussien.p@sci.asu.edu.eg (D.H.E.) Magdy_hassan@sci.asu.edu.eg (M.M.),
2 Microbial Chemistry Department, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, 12622, Giza, Egypt as.abdelgalil@nrc.sci.eg (A.S.A.-R.)
3 Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Al Kasr El-Aini, Cairo 11562, Egypt; Emad.basalious@pharma.cu.edu.eg (E.B.B.) http://orcid.org/0000-0002-0594-7119
4 Biochemistry Department, Faculty of Pharmacy, Ain Shams University, Abassia, Cairo 11566, Egypt nadia_hamdy@pharma.asu.edu.eg (N.M.H.) https://orchid.org/0000-0003-2105-107X

Abstract

Background

Acetaminophen that was highly consumed during Covid-19 recent pandemic mandates more solutions to get rid of its traces in the surrounding environment. This could be achieved via “bioremediation” using inhabitants bacterial strains present in the near soil or water environment, being a green, economic, and environment-friendly option to consider for such problem in the long-post-covid-era. If such attempt proves potential, would present a step-toward recommending “bacterial bioremediation” use at the domestic drainage, to guard against the long-term exposure to trace pharmaceuticals that impacts our (epi)genetics adversely.

Methods

First, to isolate some potential acetaminophen/paracetamol bio-degrading bacterial stain(s) from marine and terrestrial sources, through screening different Egyptian habitats,then test the
biodegrading efficacy of those strains on Media contain Acetaminophen.
Secondly, genetic Identification of those strains and chemical identification of byproducts produced from those bacteria after degrading Acetaminophen.
Finally,Chemo-informatics & Safety Examination of the Potential Biologically Active Biodegradation Products were done.

Results

Two novel bacterial strains Streptomyces Chrestomyceticus and Streptomyces Flavofuscus collected from El-Natrun Valley, Egypt, water, sediment, and sand samples were isolated, taxonomically characterized via conidiophores morphologic characterization under TEM, and 16S rRNA gene sequence analysis for genotypic identification, followed by BLAST alignment. Being novel, were deposited to the NCBI genebank Feb. 13th, 2022 https://www.ncbi.nlm.nih.gov/nuccore/OM665324
https://www.ncbi.nlm.nih.gov/nuccore/OM665325. Chemical structure identification by GC/LC MS, biological antimicrobial activity of some selected acetaminophen degradation-products extracts and derived compounds was examined against ATCC standard test organisms (bacteria and fungi) and found to be active, having good anti-microbial potential, that mandates further experimental validation.

Conclusions

After isolation of several bacterial strains from different Egyptian habitats (desert, lakes water, and soil samples) 2 novel strains with the ability to bio-degrade standard amount of acetaminophen/paracetamol® presenting “trace pharmaceuticals” in our drinking water to less-toxic/safe bio-degradation products as well as being potentially biologically active, having good anti-microbial potential, that mandates further experimental validation. We deposited these 2 novel strains to the NCBI GeneBank Feb.13th, 2022, after characterization and identification.